在线脱气机接于HPLC的流动相存储液瓶与高压恒流泵之间。流动相经由在线脱气机的入口进入脱气机中，在独立的单元真空泵腔内，通过预先设定的真空度，真 空泵连续低速运行，HPLC流动相在泵的作用下流经真空腔内一段很短的; AF脱气膜管，按照Henry’s定律，在真空作用下，溶剂中溶解的气体通过管壁排出。配了在线脱气机的液相色谱仪再不需要人工超声脱气或氦气脱气。下面我们来了解一下在线脱气机。
The online degasser is connected to the HPLC mobile phase storage tank and the high pressure constant current pump. The mobile phase enters the degasser through the entrance of an on-line degasser. In the independent unit vacuum pump cavity, the vacuum pump runs at a low speed through the predetermined vacuum degree. The HPLC flow phase flows through the true cavity in a short period of the cavity; the AF degassing membrane tube is under the action of the vacuum, in the solvent, in the solvent, in the solvent, in the solvent, in the solvent, in the solvent, and in the solvent, in the solvent, in the solvent, in the solvent. The dissolved gas is discharged through the wall of the tube. There is no need for ultrasonic degassing or helium degassing with the on-line degasser. Let's get to know the online degasser.
Q: what are the characteristics of online degassing machines for popular brand liquid chromatograph?
Answer: at present, most of the online degassing machines of popular brands use TeflonAF degassing technology. Some popular brands offer TeflonAF technology degassing machines and other types of degassing machines for users to choose, because prices vary.
Question: how to discriminate is the TeflonAF technology degasser.
Because of the high degassing efficiency of TeflonAF technology, the volume of TeflonAF degassing pipeline is very small, only less than 500ul per road. The pipeline of the ordinary degasser has 12ml. The difference is huge. This indicator is indicated in the performance indicators of the inspection apparatus.
Q: is the liquid chromatograph generally equipped with the on-line degassing machine of the original plant, or can it be matched with other brands of degassing machines?
Answer: if the original plant is equipped with TeflonAF online degassing machine, then the degassing effect will be very good. But one problem is that the price of the repair parts is too high to be accepted by the popular brand liquid chromatograph manufacturers. For example, vacuum pump and degassing box need to be replaced in case of failure. These accessories are less than 5 thousand, more than 10000 yuan, it is difficult to accept. The vacuum pump and degassing box of the on-line degasser are much cheaper. They are all imported, and their performance and life span are very high. Because the on-line degasser is independent, the on-line degasser can match all brands of liquid chromatograph, such as Waters, Agilent, SHIMADZU, Diane. Therefore, the purchase of a new liquid chromatograph with the original degasser is not necessarily the best choice. It is more suitable for an online degasser.
Q: does the four yuan liquid chromatograph with helium degassing need to be equipped with on-line degasser?
Answer: helium gas degassing is also good. It's very difficult to buy helium again after use. If there is any money, buy an on-line degasser.
Question: should two yuan double pump liquid chromatograph be equipped with on-line degassing machine?
Answer: two yuan liquid chromatograph belongs to high pressure mixed type. If the requirement is not high, it can not match the on-line degasser, but the degassing machine should be degassed before the experiment. The mobile phase after degassing can be poured into the mobile phase bottle. It's a bit of a trouble. In the case of high demand, for example, 1) the mixture ratio of the mobile phase is approximately equal, such as organic phase (methanol): water is 50:50, or 60:40 and so on, when the gas produces more, the pressure will fluctuate greatly. 2) baseline noise affects experimental results and requires lower baseline noise. Under these circumstances, the fault of the pump and detector itself is eliminated. The solution is to match the on-line degassing machine.
Q: the degassing machine of the liquid chromatograph is out of order, so we need to replace the degassing box. How do we deal with it?
Answer: because the price of the original degassing box is set too high, the replacement cost is too high. It is suggested that the online degasser should be purchased on the basis of the purchase. To solve this problem once and for all. If it is a Waters high performance liquid chromatograph, you can also use the degassing box instead of the original one, and the price is much lower. There is no problem for installation. Because the interface and the shape are exactly the same.