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  • 液相色谱的头号武器:在线脱气机
  • 本站编辑:上孰机电设备(上海)有限公司发布日期:2017-11-09 09:43 浏览次数:

般脱气机的原理是,流动相通过真空腔内的一段半透膜的管路,半透膜上面的小的孔道可以让气体分子透过而液体分子无法透过,这样流动相里面溶解的气体分子就被外面的真空从管道里面吸出来。经过脱气的流动相继续运动到泵。

The principle is like degassing machine, mobile phase through a pipeline vacuum chamber a semipermeable membrane, membrane above the small pores can make gas molecules through the liquid molecules cannot penetrate the gas molecules, such as mobile phase which dissolved were on the outside of the vacuum pipe from the inside out to. The degassed mobile phase continues to move to the pump.

QQ截图20171109094202.jpg

脱气机的使用非常简单,基本都是开了电源就自动工作。它本身有自己的一套工作流程,以保证真空腔内保持一定的真空度,持续对流动相进行脱气。

The use of degassing machine is very simple, basically open the power supply automatically work. It has its own set of working processes to ensure a vacuum in the vacuum chamber, and to continue the degassing of the mobile phase.

但是有时候如果操作不当,也可能造成脱气机问题。

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But sometimes, if improperly handled, it may also cause the problem of degassing machine.

比如,一定要在使用后,把脱气机内缓冲盐冲洗干净,然后用100%的甲醇或者乙腈充满。

For example, must be in use, the degassing machine buffer salt rinse, and then filled with 100% methanol or acetonitrile.

如果水或者缓冲盐在脱气机管路里面停留一段时间,就有可能有盐析出或者真菌的生长,造成脱气机管路的孔道堵塞。后果是脱气机仍然在工作,但是由于气体分子无法通过孔径到真空腔中,流动相的脱气效果会大打折扣。而且,真菌随着流动相进入整个液相系统,可能造成严重的污染(Contamination),干扰正常的检测。

If the water or buffer salt stays in the degassing machine pipe for a period of time, it may be salted out or fungal growth, resulting in the blockage of the degassing machine pipeline hole. The consequence is that the degassing machine is still working, but the degassing effect of the mobile phase will be greatly reduced due to the fact that the gas molecules can not pass through the pore diameter into the vacuum chamber. In addition, the fungus enters the whole liquid phase with the mobile phase, which may cause serious pollution (Contamination) and interfere with the normal detection.